Development of optimized therapeutic protocols.Study on genetic epidemiology and animal species breeding.Improved reprogramming efficiency of iPSC by using serum, serum replacement cytokines and small molecule compounds in the culture medium. Reprogramming caprine somatic cells to induce caprine iPSCs using the mRNA of multifunction inducible factors.Reprogramming ovine adult fibroblasts to pluripotency by drug-induced expression of specific factors.Reprogramming ovine fetal fibroblasts using a Dox-inducible lentiviral vector system to generate ovine iPSCs.We provide our customers with the following technical support: To generate safe ovine and caprine iPSC lines with stable inheritance, we have developed advanced and efficient strategies to reprogram ovine and caprine somatic cells. At present, several studies have reported iPSCs from ovine and caprine somatic cells, which provide powerful tools for expanding the applications of gene-modified animals.įig.1 Doxycycline controlled Tet-on-inducible lentiviral system for induction of pluripotency in sheep fetal fibroblasts. iPSC is reported to be similar to ESC in gene expression, pluripotency, and epigenetic status, and shows great potential for in vitro disease modeling and regenerative medicine. Ovine and caprine ESC technology provides excellent large-scale animal models for research on human stem cells, but it is still difficult to isolate and culture ESC from hoofed animals. In addition, ovine and caprine are considered to be important animal model species to study human diseases because they share more phylogenetic features with humans than rodents. The role of caprine genetic engineering in the pharmaceutical industry has been proved, including the production of recombinant proteins for research and human pharmaceuticals, or even in advanced clinical trials. For example, the establishment of ovine ESCs can be used to produce gene-targeted ovine with required characteristics, and improve the efficiency of somatic nuclear transfer by producing pluripotent stem cells. The establishment of embryonic stem cells (ESCs) from ungulates is very promising in many applications. Our first-class cell reprogramming technology will promote the application of ovine and caprine iPSCs in agricultural production and biomedical modeling. Creative Bioarray is committed to providing customers with efficient and advanced iPS technology to generate ovine and caprine iPSCs. Pluripotent stem cells are essential to produce accurate genetically modified animals. Ovine and caprine are important small ruminants.
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